In cybersecurity, Intrusion Detection Systems (IDS) protect against emerging cyber threats. Combining signature-based and anomaly-based detection methods may improve IDS accuracy and reduce false positives. This research analyzes hybrid intrusion detection systems signature-based components performance and limitations. The paper begins with a detailed history of signature-based detection methods responding to changing threat situations. This research analyzes signature databases to determine their capacity to identify and guard against current threats and cover known vulnerabilities. The paper also examines the intricate relationship between signature-based detection and anomalybased techniques in hybrid IDS systems. This investigation examines how these two methodologies work together to uncover old and new attack strategies, focusing on zero-day vulnerabilities and polymorphic malware. A diverse dataset of network traffic and attack scenarios is used to test. Detection, false positives, and response times assess signature-based components. Comparative examinations investigate how signature-based detection affects system accuracy and efficiency. This research illuminates the role of signature-based aspects in hybrid intrusion detection systems. This study recommends integrating signature-based detection techniques with anomaly-based methods to improve hybrid intrusion detection systems (IDS) at recognizing and mitigating various cyber threats.